Review



david chan  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc david chan
    David Chan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc12817251-589-7-9
    Average 94 stars, based on 4 article reviews
    david chan - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.
    Article Snippet: .. Constructs for the production of recombinant protein were generated as follows: Using the NdeI and BamHI restriction sites, the coding sequences of H4 (aa 1–30) were cloned into pET28-Mff(1-61)-PP-GST (Addgene plasmid #73042; provided by D. Chan) and fused with a C-terminal glutathione-S-transferase (GST) tag (Davarinejad et al., 2022). .. Site-directed mutagenesis was used to create the H4R17A mutation (QuikChange II XL, Agilent Technologies).

    Recombinant:

    Article Title: Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.
    Article Snippet: .. Constructs for the production of recombinant protein were generated as follows: Using the NdeI and BamHI restriction sites, the coding sequences of H4 (aa 1–30) were cloned into pET28-Mff(1-61)-PP-GST (Addgene plasmid #73042; provided by D. Chan) and fused with a C-terminal glutathione-S-transferase (GST) tag (Davarinejad et al., 2022). .. Site-directed mutagenesis was used to create the H4R17A mutation (QuikChange II XL, Agilent Technologies).

    Generated:

    Article Title: Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.
    Article Snippet: .. Constructs for the production of recombinant protein were generated as follows: Using the NdeI and BamHI restriction sites, the coding sequences of H4 (aa 1–30) were cloned into pET28-Mff(1-61)-PP-GST (Addgene plasmid #73042; provided by D. Chan) and fused with a C-terminal glutathione-S-transferase (GST) tag (Davarinejad et al., 2022). .. Site-directed mutagenesis was used to create the H4R17A mutation (QuikChange II XL, Agilent Technologies).

    Clone Assay:

    Article Title: Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.
    Article Snippet: .. Constructs for the production of recombinant protein were generated as follows: Using the NdeI and BamHI restriction sites, the coding sequences of H4 (aa 1–30) were cloned into pET28-Mff(1-61)-PP-GST (Addgene plasmid #73042; provided by D. Chan) and fused with a C-terminal glutathione-S-transferase (GST) tag (Davarinejad et al., 2022). .. Site-directed mutagenesis was used to create the H4R17A mutation (QuikChange II XL, Agilent Technologies).

    Plasmid Preparation:

    Article Title: Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.
    Article Snippet: .. Constructs for the production of recombinant protein were generated as follows: Using the NdeI and BamHI restriction sites, the coding sequences of H4 (aa 1–30) were cloned into pET28-Mff(1-61)-PP-GST (Addgene plasmid #73042; provided by D. Chan) and fused with a C-terminal glutathione-S-transferase (GST) tag (Davarinejad et al., 2022). .. Site-directed mutagenesis was used to create the H4R17A mutation (QuikChange II XL, Agilent Technologies).



    Similar Products

    david chan  (Addgene inc)
    94
    Addgene inc david chan
    David Chan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc12817251-589-7-9
    Average 94 stars, based on 1 article reviews
    david chan - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    pet28 mff 1 61 pp gst  (Addgene inc)
    94
    Addgene inc pet28 mff 1 61 pp gst
    Pet28 Mff 1 61 Pp Gst, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc12817251-442-10-19
    Average 94 stars, based on 1 article reviews
    pet28 mff 1 61 pp gst - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    mff 1 61  (Addgene inc)
    94
    Addgene inc mff 1 61
    Mff 1 61, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc10010011-339-30-35
    Average 94 stars, based on 1 article reviews
    mff 1 61 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    pet28 mff 1 61 pp gst vector  (Addgene inc)
    94
    Addgene inc pet28 mff 1 61 pp gst vector
    KEY RESOURCES TABLE
    Pet28 Mff 1 61 Pp Gst Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc10010011-339-33-35
    Average 94 stars, based on 1 article reviews
    pet28 mff 1 61 pp gst vector - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    pet28-mff(1–61)-pp-gst vector  (Addgene inc)
    90
    Addgene inc pet28-mff(1–61)-pp-gst vector
    KEY RESOURCES TABLE
    Pet28 Mff(1–61) Pp Gst Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mff+1+61+pp+gst/pet28+mhl/10__1016_slash_j__chb__2022__107519-603-48-50
    Average 90 stars, based on 1 article reviews
    pet28-mff(1–61)-pp-gst vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum

    doi: 10.1016/j.celrep.2023.112028

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: To generate GST-fusion proteins, the corresponding DNA fragments encoding different domains of SAYSD1 were either inserted into the pET41a vector to construct N-terminal GST fusion proteins or used to replace Mff(1–61) in the pET28-Mff(1–61)-PP-GST vector (Addgene #73042) to generate C-terminal GST-fusion proteins.

    Techniques: Virus, Recombinant, Ubiquitin Proteomics, Enzyme-linked Immunosorbent Assay, Negative Control, Software